Original Article

Frequency of Human Papillomavirus in Larynx Squamous Cell Carcinoma, Clinicopathological Correlation of p53 and Ki-67 Expressions

  • Mevlüt Çetin
  • Nesibe Kahraman Çetin
  • Sevin Kırdar
  • M. Gökhan Erpek
  • İbrahim Meteoğlu

Meandros Med Dent J 2013;14(3):13-20


Human Papillomavirus (HPV) infections have been discussed with regard to laryngeal carcinogenesis recent years. This study investigated the presence of HPV in larynx squamous cell carcinomas (SCC) via immunohistochemical and Polymerase chain reaction (PCR) array hybridization method and the relationship between p53, Ki-67 expression with HPV presence and clinicopathologic parameters.


47 patients who had been operated for larynx SCC were retrospectively included in the study. PanHPV, HPV-16, HPV-18, p53 and Ki-67 immunohistochemical stains were applied to crosssections obtained from waxed blocks by means of Avidin-Biyotin complex system. DNA was isolated from the cross-sections. The presence of HPV was investigated via a HybriBio HPV GenoArray diagnostic kit.


Polyclonal HPV positivity was detected only in 15 (31.9%) cases by means of PCR-array hybridization method. Contrary to the literature, HPV-68 was found in two cases. In HPV (PCR)-positive cases, Ki-67 staining was found at high grade (p=0.01). The expression of p53 (p=0.041) increases as differentiation decreases in tumors. When comparing Ki-67, panHPV (p=0.004) and HPV-16 (p=0.002) staining, the expression of Ki-67 is higher than others in positive cases.


Formalin-fixed paraffin-embedded tissues can be used to indicate the presence of HPV. The use of the HybriBio HPV GenoArray test was much more straightforward than other methods and is useful in detecting HPV-DNA. In the literature, no HPV-68 virus was detected in larynx SCCs until recently. The expression of Ki-67 and p53 could be central to prognosis in larynx SCCs.

Keywords: Human papillomavirus, larynx, PCR, p53, Ki-67, immunohistochemistry