Clinical Investigation

Identification and Antifungal Susceptibilities of Candida Species Isolated from Various Clinical Specimens

  • M. Cem SIRIN
  • Rabia CAN
  • Mustafa DEMIRCI

Received Date: 24.12.2003 Accepted Date: 29.04.2004 Meandros Med Dent J 2004;5(3):33-36

Objective: The aim of this study was to identify and determine antifungal susceptibility patterns of 38 yeast strains isolated from various clinical specimens. Materials and Methods: Identification of the strains were determined by API ID 32 C kit and antifungal susceptibilities of these species to flucytosine, amphotericine B, nystatin, miconazole, econazole and ketoconazole were determined by APIATB Fungus kit. Results: Of the 38 strains, 18 were isolated from urine, 8 from blood, 8 from sputum, 2 from abscess 1 from vagina and 1 from gastric contents. Candida albicans (31, 81.3%) was the most frequently isolated species, followed by C. glabrata (5, 13.2%), C. tropicalis (1, 2.6%), and C. parapsilosis (1, 2.6%). The antifungal resistance ratios of the strains were as follows; flucytosine 2.6%, amphotericine B 2.6%, nystatine 2.6%, miconazole 2.6%, econazole 47% and ketokonazole 45%. Conclusion: High rates of resistance against econazole and ketokonazole were detected in C. albicans and C. glabrata species. It is suggested that for proper treatment of Candida species infections, identification and determinations of antifungal susceptibility pattern are mandatory.Species definition and determination of antifungal susceptibility pattern are adviced for the proper treatment ofCandida infections.

Keywords: yeast, typing, in vitro antifungal susceptibility