Sıtkı Koçman University Faculty of Medicine, Department of Endocrinology and Diseases of Metabolism, Muğla, Turkey**
Sıtkı Koçman University Faculty of Medicine, Department of Obstetrics and Gynecology, Muğla, Turkey***
Sıtkı Koçman University Faculty of Medicine, Department of Internal Medicine, Muğla, Turkey****
Sıtkı Koçman University Faculty of Medicine, Department of Genetics, Muğla, Turkey
In this study, we aimed to investigate the relationship between SEPP1 gene polymorphism and gestational diabetes mellitus (GDM).
The study included 40 patients with gestational diabetes mellitus (GDM group) and 40 healthy pregnant women (control group). Target single nucleotide polymorphisms (SNPs) were studied by using rs4987017, rs13154178, rs146125471, rs28919926, rs16872762, and TaqMan probes via ABI Prısm StepOnePlus Real Time System (Applied Biosystems, Foster City, CA).
rs4987017 gene polymorphism was found to be AA homozygous in all subjects in GDM and control groups. There was no significant difference in rs146125471, rs28919926, and rs16872762 polymorphisms between GDM and control groups (p=0.656, p=0.30, and p=0.627, respectively). However, a significant difference was detected in rs13154178 polymorphism between the two groups (p<0.01). In the control group, 61.5% of subjects were AA homozygous, while there was no AA homozygous patient in the GMD group. When mutants and AA homozygous patients were compared, fasting blood glucose and blood glucose level on hour one of 50 g OGTT were found to be significantly higher in patients with polymorphism than those without (p<0.001 and p=0.01, respectively). When effects of rs13154178 gene polymorphism on lipid levels were considered, it was found that LDL cholesterol, triglyceride, and total cholesterol levels were significantly lower in AA homozygous patients than in those carrying mutant gene (p=0.036, p=0.009, and p=0.006, respectively).
To the best of our knowledge, this is the first study investigating SEPP1 gene polymorphism in GDM. Our study suggests that rs13154178 gene polymorphism lead to predisposition to GDM in pregnant Turkish women.