Clinical Investigation

The Effect of Electromagnetic Field (50 HZ, 6mT) on Rat Liver and Kidney

  • Semra ERPEK
  • Mehmet Dincer BILGIN
  • Füruzan Kaçar DOGER

Meandros Med Dent J 2007;8(1):5-11

Objectives: In the last few decades, various investigations have been performed in order to determine the possible biological effects of electromagnetic fields (EMF) generated by power lines and telecommunication technologies. The long-term exposure toEMFmay have been linked to various disorders and may affect different organs. The aim of this study was to investigate the effect of 6 millitesla (mT)AC (alternate current) EMF on the rat liver and kidney. Methods: Twenty adult maleWistar rats were divided into 2 groups; one group was exposed to 50 Hz, 6 mTAC electromagnetic field for 8 weeks, 2 hours per day, and the other sham exposed, meaning that all other experimental conditions were the same except for EMF exposure. After 8 weeks, all subjects were sacrificed, livers and kidneys were removed and weighed. For histopathological examination, samples were fixed in 10% buffered formalin, embedded in paraffin, and then cut and stained with hematoxylin-eosin, PAS and Masson's trichrome. The histological samples were evaluated using the semi-quantitative scoring. Mann Whitney U test was applied for statistical analysis. Results: For each animal the difference in body weight was calculated from the weight at the beginning of the experiment (day 0) and the weight at termination (day 56). The body weight gain of exposed group was less than that of the control group, but there was no significant difference between the two groups. The absolute and relative liver and kidney weights did not show any significant difference between the EMF exposed and sham exposed rats. Microscopic examination of liver and kidney samples revealed no significant differences between the EMF exposed and sham exposed rats. Conclusion: Present results show that whole body exposure to 50 Hz, 6 mT AC EMF doesn't cause gross and histological lesions on rat liver and kidney attributed to magnetic field exposure.

Keywords: Electromagnetic field, liver, kidney, histology