Experimental Investigation

The Effect of Carnosine on Lung Injury Induced by Abdominal Aortic Ischemia-Reperfusion

  • Bilge ALAÇAM
  • Rauf Onur EK
  • Yüksel YILDIZ
  • Mukadder SERTER
  • Nezihe Tülün BOYLU
  • Sadun TEMOÇIN

Received Date: 11.02.2010 Accepted Date: 16.04.2010 Meandros Med Dent J 2010;11(3):41-47

OBJECTIVE:

The purpose of this study is to examine the effect of carnosine on ischemia reperfusion injury in lungs occuring after occlusion reperfusion of infrarenal abdominal aorta in rats.

MATERIALS and METHODS:

Twentyfour Sprague-Dawley rats were randomly divided into 3 groups (n=8). Sham laparotomy (control) group (Group 1) underwent laparotomy and dissection of the infrarenal abdominal aorta without occlusion. In aortic ischemia reperfusion groups (group 2 and 3), after laparotomy and exteriorization of the infrarenal abdominal aorta, aorta was occluded for 30 min ischemia and then the clamp was removed for 60 min reperfusion. In group 3; 250mg/kg carnosine, were given intraperitoneally 10 min before declamping aorta. The lung tissue specimens were taken for biochemical analysis and immunohistochemical examination.

RESULTS:

In lung specimens, malonil dialdehyde, oxidized glutathione and total glutathione levels, and superoxide dismutase and myeloperoxidase activities were significantly higher in the aortic ischemia reperfusion (group 2) than the sham laparotomy (control) group (p<0.05). Levels of malonil dialdehyde and oxidized glutathione were significantly lower in the aortic ischemia reperfusion and carnosine group (group 3) than the aortic ischemia reperfusion one (p<0.05). While iNOS immunoreactivities were severe in group 2 and mild in group 3, they were not observed in group 1.

CONCLUSION:

Carnosine was beneficial in prevention of lung injury after abdominal aortic occlusionreperfusion injury.

Keywords: Ischemia reperfusion injury, lung, carnosine