Clinical Investigation

Kolorektal Adenomlar ve Adenokarsinomlarda Hücre Siklusunu Düzenleyen Proteinlerin ve Apopitozla Ilgili Belirteçlerin Ekspresyonu

  • Nil ÇULHACI
  • İbrahim METEOĞLU
  • Emel ÜNAL
  • Esra ÖZKARA
  • Hedef ÖZGÜN
  • Nezih MEYDAN
  • Muhan ERKUŞ

Received Date: 23.10.2009 Accepted Date: 27.02.2010 Meandros Med Dent J 2010;11(3):9-14

OBJECTIVE:

Tumor growth is regulated by a balance between proliferation, growth arrest and cell death. In this study, we have examined the expression of p27, cyclin D1, bcl-2, and bcl-x for evaluation of their roles in colon carcinoma progression.

MATERIALS and METHODS:

The levels of p27, cyclin D1, bcl-2, and bcl-x expression were examined by immunohistochemistry in transitional normal mucosa adjacent to adenomas (n=30), adenomas (n=30), transitional normal mucosa adjacent to adenocarcinomas (n=63), adenocarcinomas (n=63) and metastasis (n=16). Standard streptavidin-biotin immunperoxidase method was used for immunostaining and the stained slides were examined microscopically using semiquantitative criteria.

RESULTS:

Normal mucosa expressed p27 protein and adenocarcinomas displayed a decrease in the expression of this protein. Decreased expression of p27 was associated with tumor progression (p=0.026). Cyclin D1 staining was prominent in most of the adenocarcinomas and metastasis (p=0.042). Meanwhile, we could not find any relation between p27 and cyclin D1 expression. Bcl-2 and bcl-x expression also did not show any statistically significant correlation in tumor progression.

CONCLUSION:

The results of this study indicate that reduced p27 and cyclin D1 protein levels play an important role in progression of colon cancer. Bcl-2, and bcl-x expression were of no role.

Keywords: Colon carcinoma, cell cycle, apoptosis