Clinical Investigation

Kolorektal Adenomlar ve Adenokarsinomlarda Hücre Siklusunu Düzenleyen Proteinlerin ve Apopitozla Ilgili Belirteçlerin Ekspresyonu

  • İbrahim METEOĞLU
  • Emel ÜNAL
  • Esra ÖZKARA
  • Hedef ÖZGÜN
  • Nezih MEYDAN
  • Muhan ERKUŞ

Received Date: 23.10.2009 Accepted Date: 27.02.2010 Meandros Med Dent J 2010;11(3):9-14


Tumor growth is regulated by a balance between proliferation, growth arrest and cell death. In this study, we have examined the expression of p27, cyclin D1, bcl-2, and bcl-x for evaluation of their roles in colon carcinoma progression.


The levels of p27, cyclin D1, bcl-2, and bcl-x expression were examined by immunohistochemistry in transitional normal mucosa adjacent to adenomas (n=30), adenomas (n=30), transitional normal mucosa adjacent to adenocarcinomas (n=63), adenocarcinomas (n=63) and metastasis (n=16). Standard streptavidin-biotin immunperoxidase method was used for immunostaining and the stained slides were examined microscopically using semiquantitative criteria.


Normal mucosa expressed p27 protein and adenocarcinomas displayed a decrease in the expression of this protein. Decreased expression of p27 was associated with tumor progression (p=0.026). Cyclin D1 staining was prominent in most of the adenocarcinomas and metastasis (p=0.042). Meanwhile, we could not find any relation between p27 and cyclin D1 expression. Bcl-2 and bcl-x expression also did not show any statistically significant correlation in tumor progression.


The results of this study indicate that reduced p27 and cyclin D1 protein levels play an important role in progression of colon cancer. Bcl-2, and bcl-x expression were of no role.

Keywords: Colon carcinoma, cell cycle, apoptosis