Original Article

Effect of Resveratrol on microRNA Profile and Apoptosis in HL-60 Leukemia and Raji Lymphoma Cells

10.4274/meandros.2369

  • Kemal Ergin
  • Gökay Bozkurt
  • Cankut Çubuk
  • Safiye Aktaş

Received Date: 07.07.2015 Accepted Date: 20.07.2015 Meandros Med Dent J 2015;16(3):83-90

Objective:

The purpose of this study was to determine the microRNA (miRNA) profiles and the apoptosis rates in the HL-60 (promyelocytic leukemia) and Raji (Burkitt’s lymphoma) cell lines after resveratrol administration.

Materials and Methods:

The experimental design included four cell culture groups: group 1: HL-60 (control), group 2: HL-60 + resveratrol, group 3: Raji, and group 4: Raji + resveratrol. Then, two repeated microarray analyses were performed. The different miRNA expressions were identified by bioinformatic analysis and after that, validation was performed by simultaneous polymerase chain reaction. Besides, apoptosis ratio was determined with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunohistochemical markers.

Results:

By microarray analysis, eight miRNAs (hsa-mir18 to, hsa-let-7d, hsa-let- 7b, hsa-mir-1246, hsa-miR-320b, hsa-miR-92a, hsa-miR-609 and hsa-miR-337-3p) which have different expression levels between the HL-60 (control) and HL60 + resveratrol groups were detected. There was one miRNA (hsa-miR-378) which was differently expressed between Raji (control) and Raji + resveratrol groups. Four miRNAs (miR-18a, miR-92a, miR-320a, miR-378) expression levels were confirmed with simultaneous polymerase chain reaction for validation. The apoptosis (TUNEL) rate of HL-60 + resveratrol group was two times higher compared to the HL-60 control group. There was no significant difference in apoptosis (TUNEL) rate between the groups of Raji control and Raji + resveratrol. However, it showed a tendency to increase in resveratrol-administrated Raji cells. However, in the immunohistochemical analysis, especially Fas and caspase markers were more prominently expressed.

Conclusion:

In this study; effects of resveratrol, natural bioactive substances, on HL-60 and Raji cells were evaluated and different miRNA expression levels were detected. In addition, cell death was thought to be especially through the caspase and Fas mechanism. However, considering their therapeutic potential in leukemia and lymphoma further functional and organism studies are needed.

Keywords: Resveratrol, microRNA, apoptosis, leukemia, lymphoma

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