Original Article

Comparison of Two Morphometric Linear Methods to Measure the Alveolar Bone Loss Induced by Experimental Periodontal Disease in Rats

10.4274/meandros.galenos.2020.48615

  • Mehtap Bilgin Çetin
  • Yasemin Sezgin

Received Date: 09.06.2020 Accepted Date: 31.08.2020 Meandros Med Dent J 2020;21(3):190-195

Objective:

The aim of this study was to compare the direct and indirect linear morphometric measurements for the evaluation of alveolar bone loss after experimentally induced periodontitis in rats.

Materials and Methods:

Right mandibles of 30 Sprague Dawley rats were collected from a previous study which induced periodontitis using ligature model. In direct measurement group, the distance between cementoenamel junction and alveolar bone crest was evaluated by digital caliper and periodontal probe with a dental loop, whereas indirect group measurement was performed by an image analysis program on digital photographs.

Results:

The difference between groups was determined using Mann-Whitney U test. Average alveolar bone loss was 1.08±0.22 mm in direct measurement group and 1.19±0.33 mm inindirect measurement group. The variation between direct and in direct groups was not statistically significant (p>0.05).

Conclusion:

Based on the present result, it can be concluded that, direct morphometric analysis might be an essential alternative to indirect morphometric evaluations to measure alveolar bone loss after experimentally induced periodontitis in rats.

Keywords: Alveolar bone loss, experimental periodontitis, morphometric measurements, rats

Introduction

Periodontitis is a chronic inflammatory disease which yields damages in the supporting tissues of the teeth such as periodontal ligament, alveolar bone and gingiva. Parameters including host response, genetic and environmental factors play critical roles in the development of periodontal diseases and their progression (1). Since it is not always possible to detect these parameters in humans due to confounding factors or ethical problems, researchers have turned to experimental studies in various animal models (2).

Animal such as mice, hamsters, rats, minks, dogs, cats, wolves, foxes, sheep and horses, along with different non-human primates are mainly used for laboratory experiments. Primates are considered to be the best animal model since they are close relatives of humans, however these animals are not preferred in large scale studies due to their high costs. Rats are frequently preferred in experimental periodontitis studies due to multiple advantages including being cheap, easy to obtain and easy to use (3,4).

There are several methods of inducing periodontal disease in rats, such as localized injection of inactive bacteria or pathogen component, oral gavage and ligature model (5). The most commonly utilized method to induce experimental periodontitis is the ligature model which causes plaque accumulation and colonization through placing ligatures on the cervical portion of the second molar teeth of maxillary or first molar teeth of mandibular (6-11).

After the induction of periodontitis, quantifying the destruction is critical to figure out the effect of the factors investigated in the disease development. For this reason, multiple measurement methods such as morphometric, histological and microcomputed tomography (micro-CT) have been utilized to detect the loss of bone in experimentally triggered periodontitis models (12). In a previous research conducted by Li and Amar (13), no difference was detected in the accuracy of parameters including morphometric, histometric and micro-CT analysis. Similarly Fernandes et al. (7) evaluated the procedures with morphometric and histometric bases and represented that these techniques extensively detected alveolar bone loss in rats.

Morphometrical evaluation usually consists of linear and area measurements of alveolar bone loss. Morphometric evaluation is performed by a special software program using digital photographs (indirect). Another technique to linearly measure the alveolar bone loss is direct visual method which refers to the measurement made by a dental loop and digital caliper over the alveolar bone. By this way, the necessity of using of a software program and digital camera is eliminated. Therefore, this study was conducted to compare direct and indirect linear measurements to assess the alveolar bone loss. The tested hypothesis declare that direct linear measurement would be similar to indirect linear measurement to evaluate the alveolar bone loss following experimentally triggered periodontitis in rats by ligatures.


Materials and Methods


Animals

Thirty right mandibles were collected from a previous study in which periodontitis was induced in 63 male Sprague Dawley rats weighing average of 300-400 grams. Department of Medical Science Application and Research Centre of Başkent University has provided the rats. This research has been approved by Başkent University Institutional Review Board (decision no: 16/29, date: 11.04.2016) and supported by Başkent University Research Fund. During the experimental period, the housing rooms of the rats were around 22±1 °C temperature, along with 12 hours of light/dark cycle. The rats were fed ad libitum using commercially available rat chow and water. All research techniques were authorized by Governmental Animal Protection Committee and the Animal Ethics Committee of Başkent University. The research was carried out in agreement with the Helsinki Declaration.


Periodontitis Induction and Specimen Preparation

General anesthesia was performed intraperitoneally using solution containing ketamine and 2% Xylazine in 50 mg/kg and 8 mg/kg concentrations, respectively. Following general anesthesia, each animal was located on the operating table by leaving the rat’s mouth open in order to reach to the mandibular first molar teeth. Next, 3.0 silk ligatures (Doğsan, Trabzon, Turkey) were located by the mandibular first molars in order to trigger periodontitis. Thirty days later, the rats were decapitated. The mandibles and maxillas were collected. The fixation process of the specimens were performed using 10% formalin solution after the mandibles were defleshed using 9% sodium hypochlorite solution for 5 consecutive hours. Following the removal of soft tissue, the staining of the specimens were carried out using 1% methylene blue solution (Sigma-Aldrich, Saint Louis, MO, USA) for 1 minute to demarcate the cementoenamel junction (CEJ). The mandibles were placed in alginate impression to keep their occlusal surface parallel to the ground and a millimeter ruler was attached to the mandible as reference. Photographic images were obtained using a digital camera with Canon EOS 600D brand (Canon, Tokyo, Japan) coupled to a tripod in order to stabilize the camera in a parallel position to the floor at the minimal focal distance. A blinded and calibrated examiner has detected the alveolar bone loss through direct and indirect morphometric analysis.


Indirect Morphometric Analysis of Alveolar Bone Loss

Indirect morphometric analysis was conducted through evaluating standard digital photographs. The distance between CEJ and the alveolar bone crest (ABC) was determined at three equidistant sites for three times, utilizing an image processing software (Image J 1.34 s; US National Institutes of Health, Bethesda, MD, USA). To confirm the conversions of measurements, all samples were photographed along with millimeter ruler. The pixel measurements were turned into millimeter using the labeling on the millimeter ruler. An average value for each analysis was then obtained from each mandible. Figure 1 represents the samples and analysis performed on the buccal aspect.


Direct Morphometric Analysis of Alveolar Bone Loss (Direct Visual Evaluation)

Bone loss measurements were performed using a dental loop (Heine, Herrsching, Germany) with a magnification of x2.5, periodontal probe and a digital caliper. As in indirect analysis, alveolar bone loss measurement was performed at three sites. To measure the linear interval between CEJ and the deepest part of the defect, periodontal probe was placed at the base of the bone crest parallel to the vertical axis of the root surface. The point at the CEJ was marked using a tiny endodontic rubber ring. The distance from the periodontal probe tip to the rubber ring was recorded by digital caliper.


Statistical Analysis

For direct and indirect measurement groups, linear alveolar bone loss was determined as mean ± standard deviation. The obtained findings were investigated for normality of distribution using Shapiro-Wilk test. Since the data did not represent a normal distribution, we conducted Mann-Whitney U test for the detection of the variation between two groups. A p-value greater than 0.05 was designated as statistically insignificant in the tests performed.


Results

Results obtained from the direct and indirect linear morphometric assessments of average bone loss of alveolus are demonstrated in Figure 2. Average linear analysis of bone loss  are represented in millimeters. Mean alveolar bone loss has been found 1.08±0.22 mm indirect measurement group, whereas it was 1.19±0.33 mm in indirect measurement group. The variation between direct and indirect groups was found statistically insignificant (p=0.34).


Discussion

The goal of this current research was to assess two different linear morphometric methods for alveolar bone loss measurements after placing ligatures in order to experimentally induce periodontitis in rats. Direct (performed by a dental loop, periodontal probe and digital caliper over the alveolar bone) and indirect (performed by a special software program on digital photographs) linear morphometric measurements were performed on defleshed mandibles. It was observed that both direct and indirect linear morphometric methods could be used, since no statistically significance was obtained between these groups in terms of bone loss.

Several methods were used to induce periodontal disease in rats. In parallel with the current study, using cotton ligatures has been reported in multiple researches including the cervical part of the second molar of maxillary and first molar of mandibular. Since the cheek and tongue limits working by the mandible, some studies concentrated on inducing experimental periodontitis in maxilla (14-18). However, since periodontitis can be observed in both jaws, there are many studies using mandibles in the literature (19-24). Consistent with previous researches, periodontitis was triggered in the mandibular first molar teeth in the present study.

It was confirmed that the bone loss might be detected on short-time period after the placement of cotton ligature and the experimental procedure might differ between 7 to 60 days (10,25). Similar to the several previous studies, ligatures remained for 30 days in this present study (7,11,26,27).

Animal models have been widely used to detect the effect of risk factors which might significantly affect the initiation or development of a periodontal disease (17,18,22,28-34). Previous animal studies widely focused on the biological plausibility of the events and a limited number of them concentrated on methodological aspects which might alter the findings. For instance, Park et al. (28) suggested utilization of micro-computed tomography due to the compact agreement between examiners, increased reliability and high reproducibility. Li and Amar (13) have reported accuracy in micro-computed tomography, along with the histometric and morphometric measurements for the assessment of alveolar bone loss.

Morphometric measurements were achieved by two different methods and the comparison of linear and area methods were obtained in previous studies. Kuhr et al. (25) conducted a study to compare these two morphometric techniques to assess the loss of bone in the defleshed jaw. It was found that when a longer period of time was provided, these two methods as well did not represent a correlation. The authors declared that the linear method is suggested to be utilized when there is a minor variation in bone loss among the experimental groups. In contrast, Liberman et al. (35) found an almost perfect association between the linear and area evaluations. Similarly, de Souza et al. (36) compared linear and area techniques, yet the findings did not represent statistical significance. It was concluded that both methods were applicable for detecting alveolar bone loss.

Linear method has been extensively used for morphometric measurement of alveolar bone (31,32,37-39). Previous studies performed linear morphometric measurements to evaluate alveolar bone loss by taking standardized pictures from the specimens and using image analysis computer software thereafter. This analysis was compared previously with histometric and micro-computed tomographic analyses in order to quantify alveolar bone loss and it was concluded that both are adequate enough to evaluate bone loss in rats (7,13). Although morphometric linear technique performed on digital photographs is accurate in detecting bone loss and reproducible (13), the necessity of a digital camera and an image analysis software is among the disadvantages of this technique. There is only one study in the literature that compared direct and indirect methods of the distance from the junction of cemento-enamel to the bone crest of alveolus measurements. Bakır and Ay (40) reported no significant difference in measurements in two groups. Due to the scarce of the literature in this field, current study compared direct and indirect morphometric analysis to measure alveolar bone loss in rats. According to findings of the current research, direct linear morphometric measurement might be considered as an easier method without a need for a digital camera or image analysis software to address the bone loss following experimentally triggered periodontitis in rats.


Conclusion

The current research includes the use of direct morphometric analysis which might be a substitute of indirect morphometric analysis to measure alveolar bone loss in rats after the induction of experimental periodontitis. Measuring without a digital camera or image analysis software is easier and requires less time, especially when the sole purpose of research is to address bone loss. Additional research is demanded to validate the findings of present study.


Ethics

Ethics Committee Approval: This research has been approved by Başkent University Institutional Review Board (decision no: 16/29, date: 11.04.2016).

Informed Consent: This study is an animal experiment.

Peer-review: Externally peer-reviewed.

Authorship Contributions

Concept: M.B., Y.S., Design: M.B.Ç., Y.S., Supervision: M.B.Ç., Y.S., Fundings: M.B.Ç., Y.S., Materials: M.B.Ç., Y.S., Data Collection or Processing: M.B.Ç., Y.S., Analysis or Interpretation: M.B.Ç., Y.S., Literature Search: M.B.Ç., Y.S., Writing: M.B.Ç., Y.S., Critical Review: M.B.Ç., Y.S.

Conflict of Interest: No conflict of interest was declared by the authors.

Financial Disclosure: This research has been supported by Başkent University Research Fund.

Images

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